3gna

X-ray diffraction
2.4Å resolution

Crystal structure of the RAG1 nonamer-binding domain with DNA

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
V(D)J recombination-activating protein 1 Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 10.81 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P15919 (Residues: 389-464; Coverage: 7%)
Gene name: Rag1
5'-D(*AP*CP*TP*TP*AP*AP*CP*AP*AP*AP*AP*AP*CP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.23 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*TP*GP*GP*TP*TP*TP*TP*TP*GP*TP*TP*AP*AP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 14 nucleotides
Theoretical weight: 4.33 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I422
Unit cell:
a: 97.47Å b: 97.47Å c: 69.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.271
Expression systems:
  • Escherichia coli
  • Not provided