› 3glh

X-ray diffraction
3.89Å resolution

Crystal Structure of the E. coli clamp loader bound to Psi Peptide

Released:
DOI: 10.2210/pdb3glh/pdb
PDB entry 3glh coloured by chain, front view.
PDB entry 3glh coloured by chain, side view.
PDB entry 3glh coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The mechanism of ATP-dependent primer-template recognition by a clamp loader complex.
Simonetta KR, Kazmirski SL, Goedken ER, Cantor AJ, Kelch BA, McNally R, Seyedin SN, Makino DL, O'Donnell M, Kuriyan J
Cell 137 659-71 (2009)
PMID: 19450514

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139153 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA polymerase III subunit delta Chains: A, F, K
Molecule details ›
Chains: A, F, K
Length: 343 amino acids
Theoretical weight: 38.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P28630 (Residues: 1-343; Coverage: 100%)
Gene names: JW0635, b0640, holA
Sequence domains:
Structure domains:
DNA polymerase III subunit tau Chains: B, C, D, G, H, I, L, M, N
Molecule details ›
Chains: B, C, D, G, H, I, L, M, N
Length: 376 amino acids
Theoretical weight: 41.8 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06710 (Residues: 1-373; Coverage: 58%)
Gene names: JW0459, b0470, dnaX, dnaZ, dnaZX
Sequence domains:
Structure domains:
DNA polymerase III subunit delta' Chains: E, J, O
Molecule details ›
Chains: E, J, O
Length: 334 amino acids
Theoretical weight: 36.98 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P28631 (Residues: 1-334; Coverage: 100%)
Gene names: JW1085, b1099, holB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 100.162Å b: 228.494Å c: 164.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.359 0.359 0.361
Expression system: Escherichia coli

Quick links

Citations

20 review citations

DNA replicases from a bacterial perspective.
McHenry CS. (2011)
19 more

3 mentions without citation

The mechanism of ATP-dependent primer-template recognition by a clamp loader complex.
Simonetta et al. (2009)
2 more