› 3gl2

X-ray diffraction
2.1Å resolution

Crystal structure of dicamba monooxygenase bound to dicamba

Released:
DOI: 10.2210/pdb3gl2/pdb
PDB entry 3gl2 coloured by chain, front view.
PDB entry 3gl2 coloured by chain, side view.
PDB entry 3gl2 coloured by chain, top view.
Source organism: Stenotrophomonas maltophilia
Primary publication:
Crystal structure of dicamba monooxygenase: a Rieske nonheme oxygenase that catalyzes oxidative demethylation.
Dumitru R, Jiang WZ, Weeks DP, Wilson MA
J Mol Biol 392 498-510 (2009)
PMID: 19616011

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dicamba O-demethylase, oxygenase component Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 349 amino acids
Theoretical weight: 38.64 KDa
Source organism: Stenotrophomonas maltophilia
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5S3I3 (Residues: 2-339; Coverage: 100%)
Gene name: ddmC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand FES 3 x FES
Ligand FE 2 x FE
Ligand D3M 2 x D3M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P32
Unit cell:
a: 81.323Å b: 81.323Å c: 159.282Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.19 0.247
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Dioxygen activation by nonheme iron enzymes with the 2-His-1-carboxylate facial triad that generate high-valent oxoiron oxidants.
Kal et al. (2017)
2 more

4 mentions without citation

Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids.
Summers et al. (2012)
3 more