› 3gjn

X-ray diffraction
2.48Å resolution

Following evolutionary paths to high affinity and selectivity protein-protein interactions using Colicin7 and Immunity proteins

Released:
DOI: 10.2210/pdb3gjn/pdb
PDB 3gjn coloured by chain and viewed from the front.
PDB 3gjn coloured by chain and viewed from the side.
PDB 3gjn coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Following evolutionary paths to protein-protein interactions with high affinity and selectivity.
Levin KB, Dym O, Albeck S, Magdassi S, Keeble AH, Kleanthous C, Tawfik DS
Nat. Struct. Mol. Biol. 16 1049-55 (2009)
PMID: 19749752

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E9 immunity protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 86 amino acids
Theoretical weight: 9.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13479 (Residues: 1-86; Coverage: 100%)
Gene names: ceiE9, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E7 Chains: B, C
Molecule details ›
Chains: B, C
Length: 141 amino acids
Theoretical weight: 16.23 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47112 (Residues: 446-576; Coverage: 23%)
Gene names: cea, colE7
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 59.65Å b: 53.713Å c: 79.334Å
α: 90° β: 105.59° γ: 90°
R-values:
R R work R free
0.244 0.242 0.276
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Accuracy-rate tradeoffs: how do enzymes meet demands of selectivity and catalytic efficiency?
Tawfik DS. (2014)
6 more

1 mention without citation

Integrating energy calculations with functional assays to decipher the specificity of G protein-RGS protein interactions.
Kosloff et al. (2011)