3gjn

X-ray diffraction
2.48Å resolution

Following evolutionary paths to high affinity and selectivity protein-protein interactions using Colicin7 and Immunity proteins

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E9 immunity protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 86 amino acids
Theoretical weight: 9.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13479 (Residues: 1-86; Coverage: 100%)
Gene names: ceiE9, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E7 Chains: B, C
Molecule details ›
Chains: B, C
Length: 141 amino acids
Theoretical weight: 16.23 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47112 (Residues: 446-576; Coverage: 23%)
Gene names: cea, colE7
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 59.65Å b: 53.713Å c: 79.334Å
α: 90° β: 105.59° γ: 90°
R-values:
R R work R free
0.244 0.242 0.276
Expression system: Escherichia coli