› 3gjb

X-ray diffraction
2.2Å resolution

CytC3 with Fe(II) and alpha-ketoglutarate

Released:
DOI: 10.2210/pdb3gjb/pdb
PDB entry 3gjb coloured by chain, front view.
PDB entry 3gjb coloured by chain, side view.
PDB entry 3gjb coloured by chain, top view.
Source organism: Streptomyces sp.
Primary publication:
Structural analysis of an open active site conformation of nonheme iron halogenase CytC3.
Wong C, Fujimori DG, Walsh CT, Drennan CL
J Am Chem Soc 131 4872-9 (2009)
PMID: 19281171

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-112201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CytC3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 319 amino acids
Theoretical weight: 36.48 KDa
Source organism: Streptomyces sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VX22 (Residues: 1-319; Coverage: 100%)
Gene name: cytC3
Sequence domains: Phytanoyl-CoA dioxygenase (PhyH)
Structure domains: q2cbj1_9rhob like domain

Ligands and Environments

4 bound ligands:
Ligand FE2 2 x FE2
Ligand AKG 3 x AKG
Ligand ACT 8 x ACT
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P43212
Unit cell:
a: 88.988Å b: 88.988Å c: 248.442Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.259
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Prolyl 4-hydroxylase.
Gorres et al. (2010)
12 more

6 mentions without citation

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.
Agarwal et al. (2017)
5 more