X-ray diffraction
1.6Å resolution

Crystal Structures of the G81A Mutant of the Active Chimera of (S)-Mandelate Dehydrogenase and its Complex with Two of its Substrates


Function and Biology Details

Reaction catalysed:
(S)-2-hydroxy-2-phenylacetate + acceptor = 2-oxo-2-phenylacetate + reduced acceptor
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
(S)-mandelate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 42.15 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
  • Canonical: P20932 (Residues: 1-393; Coverage: 95%)
Gene name: mdlB
Sequence domains: FMN-dependent dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments

Cofactor: Ligand FMN 1 x FMN
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: I4
Unit cell:
a: 99.36Å b: 99.36Å c: 87.55Å
α: 90° β: 90° γ: 90°
R R work R free
0.134 0.134 0.171
Expression system: Escherichia coli