3gii

X-ray diffraction
2.6Å resolution

Dpo4 extension ternary complex with disordered A opposite an oxoG in anti conformation

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase IV Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 38.95 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97W02 (Residues: 2-341; Coverage: 97%)
Gene names: SSO2448, dbh, dpo4
Sequence domains:
Structure domains:
5'-D(*GP*TP*TP*GP*GP*AP*TP*GP*GP*TP*AP*GP*(2DA))-3' Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 4.07 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Not provided
5'-D(*CP*TP*AP*AP*CP*(8OG)P*CP*TP*AP*CP*CP*AP*TP*CP*CP*AP*AP*CP*C)-3' Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 5.69 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Not provided

Ligands and Environments

3 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 95.761Å b: 111.646Å c: 52.708Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.265
Expression systems:
  • Escherichia coli
  • Not provided