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Crystal Structure of ApcT Transporter Bound to 7F11 Monoclonal Fab Fragment

X-ray diffraction
2.48Å resolution
PDB entry 3gi9 coloured by chain, front view.
PDB entry 3gi9 coloured by chain, side view.
PDB entry 3gi9 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Uncharacterized protein MJ0609 and IG-light kappa chain and IG-heavy chain
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Multimeric state: hetero trimer
Accessible surface area: 34903.59 Å2
Buried surface area: 5356.09 Å2
Dissociation area: 921.89 Å2
Dissociation energy (ΔGdiss): 1.25 kcal/mol
Dissociation entropy (TΔSdiss): 13.75 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-212286

Macromolecules

7F11 Anti-ApcT Monoclonal Fab Light Chain
Chain: L
Length: 220 amino acids
Theoretical weight: 24.28 KDa
Source organism: Mus musculus
Expression system: Mus musculus
InterPro:
CATH: Immunoglobulins
7F11 Anti-ApcT Monoclonal Fab Light Chain in PDB entry 3gi9, assembly 1, front view.
7F11 Anti-ApcT Monoclonal Fab Light Chain in PDB entry 3gi9, assembly 1, side view.
7F11 Anti-ApcT Monoclonal Fab Light Chain in PDB entry 3gi9, assembly 1, top view.
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7F11 Anti-ApcT Monoclonal Fab Heavy Chain
Chain: H
Length: 223 amino acids
Theoretical weight: 23.98 KDa
Source organism: Mus musculus
Expression system: Mus musculus
InterPro:
CATH: Immunoglobulins
7F11 Anti-ApcT Monoclonal Fab Heavy Chain in PDB entry 3gi9, assembly 1, front view.
7F11 Anti-ApcT Monoclonal Fab Heavy Chain in PDB entry 3gi9, assembly 1, side view.
7F11 Anti-ApcT Monoclonal Fab Heavy Chain in PDB entry 3gi9, assembly 1, top view.
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Uncharacterized protein MJ0609
Chain: C
Length: 444 amino acids
Theoretical weight: 48.33 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58026 (Residues: 1-435; Coverage: 100%)
Gene name: MJ0609
Pfam: Amino acid permease
InterPro: Amino acid/polyamine transporter I
CATH: Amino acid/polyamine transporter I
Uncharacterized protein MJ0609 in PDB entry 3gi9, assembly 1, front view.
Uncharacterized protein MJ0609 in PDB entry 3gi9, assembly 1, side view.
Uncharacterized protein MJ0609 in PDB entry 3gi9, assembly 1, top view.
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