Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128311 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Global transcriptional regulator Spx
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 15.55 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
Sequence domains: ArsC family
Structure domains: Glutaredoxin
Length: 131 amino acids
Theoretical weight: 15.55 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
- Canonical:
O31602 (Residues: 1-131; Coverage: 100%)
Sequence domains: ArsC family
Structure domains: Glutaredoxin
DNA-directed RNA polymerase subunit alpha
Molecule details ›
Chain: B
Length: 79 amino acids
Theoretical weight: 9.12 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
Sequence domains: Bacterial RNA polymerase, alpha chain C terminal domain
Structure domains: 5' to 3' exonuclease, C-terminal subdomain
Length: 79 amino acids
Theoretical weight: 9.12 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
- Canonical: P20429 (Residues: 240-314; Coverage: 24%)
Sequence domains: Bacterial RNA polymerase, alpha chain C terminal domain
Structure domains: 5' to 3' exonuclease, C-terminal subdomain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X25
Spacegroup:
P212121
Expression system: Escherichia coli
