› 3gdf

X-ray diffraction
2.5Å resolution

Crystal structure of the NADP-dependent mannitol dehydrogenase from Cladosporium herbarum.

Released:
DOI: 10.2210/pdb3gdf/pdb
PDB entry 3gdf coloured by chain, front view.
PDB entry 3gdf coloured by chain, side view.
PDB entry 3gdf coloured by chain, top view.
Source organism: Cladosporium herbarum
Primary publication:
Crystal structure of the NADP-dependent mannitol dehydrogenase from Cladosporium herbarum: Implications for oligomerisation and catalysis.
Nüss D, Goettig P, Magler I, Denk U, Breitenbach M, Schneider PB, Brandstetter H, Simon-Nobbe B
Biochimie 92 985-93 (2010)
PMID: 20420880

Function and Biology Details

Reaction catalysed: 
D-mannitol + NADP(+) = D-fructose + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent mannitol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 28.5 KDa
Source organism: Cladosporium herbarum
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C0Y5 (Residues: 1-267; Coverage: 100%)
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 69.012Å b: 106.536Å c: 132.783Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.262
Expression system: Escherichia coli

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Citations

2 review citations

Mold allergens in respiratory allergy: from structure to therapy.
Twaroch et al. (2015)
1 more

4 mentions without citation

Structure of allergens and structure based epitope predictions.
Dall'antonia et al. (2014)
3 more