3gcz

X-ray diffraction
1.7Å resolution

Yokose virus Methyltransferase in complex with AdoMet

Released:
Source organism: Yokose virus
Primary publication:
Crystal structure of a methyltransferase from a no-known-vector Flavivirus.
Biochem. Biophys. Res. Commun. 382 200-4 (2009)
PMID: 19275894

Function and Biology Details

Reactions catalysed:
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Core protein Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 31.64 KDa
Source organism: Yokose virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7T918 (Residues: 2520-2784; Coverage: 8%)
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P32
Unit cell:
a: 68.912Å b: 68.912Å c: 50.806Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.167 0.198
Expression system: Escherichia coli