› 3g6k

X-ray diffraction
1.35Å resolution

Crystal Structure of Candida glabrata FMN Adenylyltransferase in complex with FAD and Inorganic Pyrophosphate

Released:
DOI: 10.2210/pdb3g6k/pdb
PDB entry 3g6k coloured by chain, front view.
PDB entry 3g6k coloured by chain, side view.
PDB entry 3g6k coloured by chain, top view.
Source organism: [Candida] glabrata
Primary publication:
Structure and mechanism of a eukaryotic FMN adenylyltransferase.
Huerta C, Borek D, Machius M, Grishin NV, Zhang H
J Mol Biol 389 388-400 (2009)
PMID: 19375431

Function and Biology Details

Reaction catalysed: 
ATP + FMN = diphosphate + FAD
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179616 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 308 amino acids
Theoretical weight: 35.99 KDa
Source organism: [Candida] glabrata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6FNA9 (Residues: 1-304; Coverage: 100%)
Gene names: CAGL0K01397g, FMNAT
Sequence domains: Phosphoadenosine phosphosulfate reductase family
Structure domains: HUPs

Ligands and Environments


Cofactor: Ligand FAD 6 x FAD
4 bound ligands:
Ligand POP 6 x POP
Ligand MG 13 x MG
Ligand SO4 4 x SO4
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 206.583Å b: 81.481Å c: 136.603Å
α: 90° β: 129.79° γ: 90°
R-values:
R R work R free
0.155 0.153 0.187
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
Abbas et al. (2011)
4 more

4 mentions without citation

Structure and mechanism of a eukaryotic FMN adenylyltransferase.
Huerta et al. (2009)
3 more