› 3g2o

X-ray diffraction
2.1Å resolution

Crystal Structure of the Glycopeptide N-methyltransferase MtfA complexed with (S)-adenosyl-L-methionine (SAM)

Released:
DOI: 10.2210/pdb3g2o/pdb
PDB 3g2o coloured by chain and viewed from the front.
PDB 3g2o coloured by chain and viewed from the side.
PDB 3g2o coloured by chain and viewed from the top.
Source organism: Amycolatopsis orientalis
Primary publication:
Structure and function of the glycopeptide N-methyltransferase MtfA, a tool for the biosynthesis of modified glycopeptide antibiotics.
Shi R, Lamb SS, Zakeri B, Proteau A, Cui Q, Sulea T, Matte A, Wright GD, Cygler M
Chem. Biol. 16 401-10 (2009)
PMID: 19389626

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 32.53 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli
UniProt:
  • Canonical: O52805 (Residues: 1-280; Coverage: 100%)
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SAM 2 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 126.917Å b: 72.335Å c: 75.306Å
α: 90° β: 103.89° γ: 90°
R-values:
R R work R free
0.218 0.216 0.245
Expression system: Escherichia coli

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Citations

9 review citations

Nonribosomal Peptide Synthesis-Principles and Prospects.
Süssmuth et al. (2017)
8 more

1 mention without citation

Nonribosomal peptides for iron acquisition: pyochelin biosynthesis as a case study.
Ronnebaum et al. (2018)