› 3g0o

X-ray diffraction
1.8Å resolution

Crystal structure of 3-hydroxyisobutyrate dehydrogenase (ygbJ) from Salmonella typhimurium

Released:
DOI: 10.2210/pdb3g0o/pdb
PDB entry 3g0o coloured by chain, front view.
PDB entry 3g0o coloured by chain, side view.
PDB entry 3g0o coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Entry authors: Eswaramoorthy S, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
L-threonate + NAD(+) = 2-dehydro-L-erythronate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-187278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-threonate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 31.69 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZMG4 (Residues: 3-294; Coverage: 95%)
Gene names: STM2918, ygbJ
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand TLA 1 x TLA
1 modified residue:
Ligand MSE 13 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: I222
Unit cell:
a: 50.513Å b: 132.416Å c: 143.067Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.207 0.233
Expression system: Escherichia coli

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