› 3fze

X-ray diffraction
1.6Å resolution

Structure of the 'minimal scaffold' (ms) domain of Ste5 that cocatalyzes Fus3 phosphorylation by Ste7

Released:
DOI: 10.2210/pdb3fze/pdb
PDB entry 3fze coloured by chain, front view.
PDB entry 3fze coloured by chain, side view.
PDB entry 3fze coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The Ste5 scaffold directs mating signaling by catalytically unlocking the Fus3 MAP kinase for activation.
Good M, Tang G, Singleton J, Reményi A, Lim WA
Cell 136 1085-97 (2009)
PMID: 19303851

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein STE5 Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32917 (Residues: 593-786; Coverage: 21%)
Gene names: NUL3, STE5, YD8557.12, YDR103W
Sequence domains: Protein kinase Fus3-binding
Structure domains: Protein Ste5, Fus3-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 46.803Å b: 63.569Å c: 69.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.237
Expression system: Escherichia coli

Quick links

Citations

39 review citations

Mechanisms and functions of p38 MAPK signalling.
Cuadrado et al. (2010)
38 more

1 mention without citation

Scaffold proteins: hubs for controlling the flow of cellular information.
Good et al. (2011)