› 3fv5

X-ray diffraction
1.8Å resolution

Crystal Structure of E. coli Topoisomerase IV co-complexed with inhibitor

Released:
DOI: 10.2210/pdb3fv5/pdb
PDB entry 3fv5 coloured by chain, front view.
PDB entry 3fv5 coloured by chain, side view.
PDB entry 3fv5 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Wei Y, Charifson P, LeTiran A

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 4 subunit B Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 22.35 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P20083 (Residues: 15-215; Coverage: 32%)
Gene names: JW2998, b3030, nfxD, parE
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Structure domains: Histidine kinase-like ATPase, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand 1EU 2 x 1EU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 85.42Å b: 72.84Å c: 70.97Å
α: 90° β: 96.41° γ: 90°
R-values:
R R work R free
0.199 0.197 0.24
Expression system: Escherichia coli

Quick links