› 3ft0

X-ray diffraction
1.8Å resolution

Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced

Released:
DOI: 10.2210/pdb3ft0/pdb
PDB entry 3ft0 coloured by chain, front view.
PDB entry 3ft0 coloured by chain, side view.
PDB entry 3ft0 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Metal-binding loop length and not sequence dictates structure.
Sato K, Li C, Salard I, Thompson AJ, Banfield MJ, Dennison C
Proc Natl Acad Sci U S A 106 5616-21 (2009)
PMID: 19299503

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 13.86 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-140, 141-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU1 2 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 34.664Å b: 86.989Å c: 42.156Å
α: 90° β: 114.67° γ: 90°
R-values:
R R work R free
0.193 0.189 0.25
Expression system: Escherichia coli

Quick links

Citations

13 citation in other articles

Enhancement of catalysis and functional expression of a bacterial laccase by single amino acid replacement.
Nasoohi et al. (2013)
12 more

1 mention without citation

Metal-binding loop length and not sequence dictates structure.
Sato et al. (2009)