› 3fsg

X-ray diffraction
2Å resolution

Crystal structure of alpha/beta superfamily hydrolase from Oenococcus oeni PSU-1

Released:
DOI: 10.2210/pdb3fsg/pdb
PDB entry 3fsg coloured by chain, front view.
PDB entry 3fsg coloured by chain, side view.
PDB entry 3fsg coloured by chain, top view.
Source organism: Oenococcus oeni PSU-1
Entry authors: Nocek B, Bigelow L, Cobb G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
homo trimer
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-170096 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AB hydrolase-1 domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 272 amino acids
Theoretical weight: 31.39 KDa
Source organism: Oenococcus oeni PSU-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04D10 (Residues: 1-269; Coverage: 100%)
Gene name: OEOE_1827
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase
AB hydrolase-1 domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 272 amino acids
Theoretical weight: 31.37 KDa
Source organism: Oenococcus oeni PSU-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04D10 (Residues: 1-269; Coverage: 100%)
Gene name: OEOE_1827
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

4 bound ligands:
Ligand EDO 7 x EDO
Ligand GOL 2 x GOL
Ligand SO4 7 x SO4
Ligand CL 2 x CL
2 modified residues:
Ligand MSE 20 x MSE
Ligand CSO 3 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 217.493Å b: 40.58Å c: 135.98Å
α: 90° β: 107.5° γ: 90°
R-values:
R R work R free
0.182 0.18 0.225
Expression system: Escherichia coli

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