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X-ray diffraction
2.15Å resolution

X-ray structure of QdtB from T. thermosaccharolyticum in complex with a PLP:TDP-3-aminoquinovose aldimine

Released:
DOI: 10.2210/pdb3frk/pdb
PDB entry 3frk coloured by chain, front view.
PDB entry 3frk coloured by chain, side view.
PDB entry 3frk coloured by chain, top view.
Source organism: Thermoanaerobacterium thermosaccharolyticum
Primary publication:
Structural analysis of QdtB, an aminotransferase required for the biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose.
Thoden JB, Schäffer C, Messner P, Holden HM
Biochemistry 48 1553-61 (2009)
PMID: 19178182

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
QdtB Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 42.68 KDa
Source organism: Thermoanaerobacterium thermosaccharolyticum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6TFC4 (Residues: 1-365; Coverage: 100%)
Gene name: qdtB
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TQP 2 x TQP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P61
Unit cell:
a: 109.1Å b: 109.1Å c: 177.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.269
Expression system: Escherichia coli

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Citations

4 review citations

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
3 more

7 mentions without citation

The s-layer glycome-adding to the sugar coat of bacteria.
Ristl et al. (2011)
6 more