3frh

X-ray diffraction
1.2Å resolution

Structure of the 16S rRNA methylase RmtB, P21

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(1405) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(1405) in 16S rRNA 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
16S rRNA (guanine(1405)-N(7))-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 27.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q763K9 (Residues: 1-251; Coverage: 100%)
Gene names: AKG29_00615, BANRA_05536, BVL39_07930, E4K51_26945, EI021_25950, IPF_158, JFD_05346, RmtB, TUM18780_46020, pHN3A11_015, pHN7A8_021, pHNFP460_047, rmtB
Sequence domains: Ribosomal RNA methyltransferase (FmrO)
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 50.856Å b: 48.304Å c: 54.558Å
α: 90° β: 115.05° γ: 90°
R-values:
R R work R free
0.139 0.139 0.159
Expression system: Escherichia coli