X-ray diffraction
2.36Å resolution

The crystal structure of a methyltransferase domain from Bacteroides thetaiotaomicron VPI

Source organism: Bacteroides thetaiotaomicron
Entry authors: Tan K, Tesar C, Cobb G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + cytidine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(1402) in 16S rRNA 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Ribosomal RNA small subunit methyltransferase I Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 12.98 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli
  • Canonical: Q8A5G6 (Residues: 113-224; Coverage: 50%)
Gene names: BT_2273, rsmI
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases
Structure domains: Methyltransferase, Cobalt-precorrin-4 Transmethylase; Domain 2

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 44.321Å b: 70.919Å c: 86.908Å
α: 90° β: 90° γ: 90°
R R work R free
0.217 0.214 0.286
Expression system: Escherichia coli