X-ray diffraction
1.82Å resolution

Saccharomyces cerevisiae THI4p is a suicide thiamin thiazole synthase


Function and Biology Details

Reaction catalysed:
NAD(+) + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Thiamine thiazole synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
  • Canonical: P32318 (Residues: 1-326; Coverage: 100%)
Gene names: ESP35, G6620, MOL1, THI4, YGR144W
Sequence domains: Thi4 family
Structure domains:

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: I4
Unit cell:
a: 140.696Å b: 140.696Å c: 73.325Å
α: 90° β: 90° γ: 90°
R R work R free
0.198 0.195 0.236
Expression system: Escherichia coli