› 3fo4

X-ray diffraction
1.9Å resolution

Crystal structure of guanine riboswitch C74U mutant bound to 6-chloroguanine

Released:
DOI: 10.2210/pdb3fo4/pdb
PDB entry 3fo4 coloured by chain, front view.
PDB entry 3fo4 coloured by chain, side view.
PDB entry 3fo4 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Adaptive ligand binding by the purine riboswitch in the recognition of guanine and adenine analogs.
Gilbert SD, Reyes FE, Edwards AL, Batey RT
Structure 17 857-68 (2009)
PMID: 19523903

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195852 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Guanine riboswitch C74U mutant Chain: A
Molecule details ›
Chain: A
Length: 68 nucleotides
Theoretical weight: 21.84 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
Sequence domains: Purine riboswitch

Ligands and Environments

3 bound ligands:
Ligand 6GU 1 x 6GU
Ligand ACT 1 x ACT
Ligand NCO 12 x NCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 132.6Å b: 35.17Å c: 41.79Å
α: 90° β: 90.38° γ: 90°
R-values:
R R work R free
0.226 0.226 0.249
Expression system: Not provided

Quick links

Citations

19 review citations

Principles for targeting RNA with drug-like small molecules.
Warner et al. (2018)
18 more

5 mentions without citation

Using RNA Sequence and Structure for the Prediction of Riboswitch Aptamer: A Comprehensive Review of Available Software and Tools.
Antunes et al. (2017)
4 more