PDBe 3fmp

X-ray diffraction
3.19Å resolution

Crystal structure of the nucleoporin Nup214 in complex with the DEAD-box helicase Ddx19

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear pore complex protein Nup214 Chains: A, C
Molecule details ›
Chains: A, C
Length: 450 amino acids
Theoretical weight: 49.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35658 (Residues: 1-450; Coverage: 22%)
Gene names: CAIN, CAN, KIAA0023, NUP214
ATP-dependent RNA helicase DDX19B Chains: B, D
Molecule details ›
Chains: B, D
Length: 479 amino acids
Theoretical weight: 53.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UMR2 (Residues: 1-479; Coverage: 100%)
Gene names: DBP5, DDX19, DDX19B, TDBP
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 61.369Å b: 112.915Å c: 142.573Å
α: 90° β: 89.86° γ: 90°
R-values:
R R work R free
0.251 0.249 0.283
Expression system: Escherichia coli