3fm7

X-ray diffraction
3.5Å resolution

Quaternary Structure of Drosophila melanogaster IC/Tctex-1/LC8; Allosteric Interactions of Dynein Light Chains with Dynein Intermediate Chain

Released:
Source organism: Drosophila melanogaster
Primary publication:
Multivalency in the assembly of intrinsically disordered Dynein intermediate chain.
J Biol Chem 284 33115-21 (2009)
PMID: 19759397

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-173174 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Dynein light chain Tctex-type Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 12.49 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94524 (Residues: 1-111; Coverage: 100%)
Gene names: CG12363, Dlc90F, Tctex
Sequence domains: Tctex-1 family
Structure domains: Tctex-1
Cytoplasmic dynein 1 intermediate chain Chains: C, D
Molecule details ›
Chains: C, D
Length: 27 amino acids
Theoretical weight: 3.05 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q24246 (Residues: 109-135; Coverage: 4%)
Gene names: CG18000, Cdic, Dic19B, sw
Sequence domains: Cytoplasmic dynein 1 intermediate chain 2
Dynein light chain 1, cytoplasmic Chains: E, F
Molecule details ›
Chains: E, F
Length: 89 amino acids
Theoretical weight: 10.39 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q24117 (Residues: 1-89; Coverage: 100%)
Gene names: CG6998, Cdlc1, ctp, ddlc1
Sequence domains: Dynein light chain type 1
Structure domains: Protein Inhibitor Of Neuronal Nitric Oxide Synthase;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P62
Unit cell:
a: 115.79Å b: 115.79Å c: 90.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.17 0.275
Expression system: Escherichia coli