X-ray diffraction
1.9Å resolution

Human dihydroorotate dehydrogenase in complex with a leflunomide derivative inhibitor 3


Function and Biology Details

Reaction catalysed:
(S)-dihydroorotate + a quinone = orotate + a quinol

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Dihydroorotate dehydrogenase (quinone), mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 39.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q02127 (Residues: 29-395; Coverage: 93%)
Gene name: DHODH
Sequence domains: Dihydroorotate dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments

Cofactor: Ligand FMN 1 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P3221
Unit cell:
a: 90.888Å b: 90.888Å c: 122.915Å
α: 90° β: 90° γ: 120°
R R work R free
0.18 0.177 0.209
Expression system: Escherichia coli