› 3ffd

X-ray diffraction
2Å resolution

Structure of parathyroid hormone-related protein complexed to a neutralizing monoclonal antibody

Released:
DOI: 10.2210/pdb3ffd/pdb
PDB entry 3ffd coloured by chain, front view.
PDB entry 3ffd coloured by chain, side view.
PDB entry 3ffd coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for antibody discrimination between two hormones that recognize the parathyroid hormone receptor.
McKinstry WJ, Polekhina G, Diefenbach-Jagger H, Ho PW, Sato K, Onuma E, Gillespie MT, Martin TJ, Parker MW
J Biol Chem 284 15557-63 (2009)
PMID: 19346515

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233982 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Monoclonal antibody, heavy chain, Fab fragment Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 23.34 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Monoclonal antibody, light chain, Fab fragment Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 23.75 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Parathyroid hormone-related protein Chain: P
Molecule details ›
Chain: P
Length: 108 amino acids
Theoretical weight: 12.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12272 (Residues: 37-144; Coverage: 71%)
Gene names: PTHLH, PTHRP
Sequence domains: Parathyroid hormone family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21212
Unit cell:
a: 72.613Å b: 96.285Å c: 88.544Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.271
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

4 citation in other articles

Linear and extended: a common polyglutamine conformation recognized by the three antibodies MW1, 1C2 and 3B5H10.
Klein et al. (2013)
3 more

13 mentions without citation

Epitopia: a web-server for predicting B-cell epitopes.
Rubinstein et al. (2009)
12 more