3fc9

X-ray diffraction
2.8Å resolution

Crystal structure of the Mimivirus NDK +Kpn-N62L double mutant complexed with CDP

Released:

Function and Biology Details

Reaction catalysed:
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside diphosphate kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 146 amino acids
Theoretical weight: 16.63 KDa
Source organism: Acanthamoeba polyphaga mimivirus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5UQL3 (Residues: 2-92, 93-137; Coverage: 99%)
Gene names: MIMI_R418, NDK
Sequence domains: Nucleoside diphosphate kinase
Structure domains: Nucleoside diphosphate kinase-like domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2221
Unit cell:
a: 82.586Å b: 153.043Å c: 184.786Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.241
Expression system: Escherichia coli