› 3f8r

X-ray diffraction
1.95Å resolution

Crystal structure of Sulfolobus solfataricus Thioredoxin reductase B3 in complex with two NADP molecules

Released:
DOI: 10.2210/pdb3f8r/pdb
PDB entry 3f8r coloured by chain, front view.
PDB entry 3f8r coloured by chain, side view.
PDB entry 3f8r coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structure and stability of a thioredoxin reductase from Sulfolobus solfataricus: a thermostable protein with two functions.
Ruggiero A, Masullo M, Ruocco MR, Grimaldi P, Lanzotti MA, Arcari P, Zagari A, Vitagliano L
Biochim Biophys Acta 1794 554-62 (2009)
PMID: 19110078

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD/NAD(P)-binding domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 323 amino acids
Theoretical weight: 35.27 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8X236 (Residues: 1-324; Coverage: 100%)
Gene name: nox35
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand NAP 8 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 76.871Å b: 123.425Å c: 127.435Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.208 0.257
Expression system: Escherichia coli

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Citations

9 citation in other articles

Molecular characterization of the thioredoxin system from Methanosarcina acetivorans.
McCarver et al. (2014)
8 more