3f2b

X-ray diffraction
2.39Å resolution

DNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP, Mg and Zn

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase III PolC-type Chain: A
5'-D(*DCP*DAP*DGP*DTP*DGP*DAP*DGP*DAP*DCP*DGP*DGP*DGP*DCP*DAP*DAP*DCP*DC)-3' Chain: P
Molecule details ›
Chain: P
Length: 17 nucleotides
Theoretical weight: 5.25 KDa
5'-D(*DAP*DTP*DAP*DAP*DCP*DGP*DGP*DTP*DTP*DGP*DCP*DCP*DCP*DGP*DTP*DCP*DTP*DCP*DAP*DCP*DTP*DG)-3' Chain: T
Molecule details ›
Chain: T
Length: 22 nucleotides
Theoretical weight: 6.7 KDa

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2221
Unit cell:
a: 116.174Å b: 140.793Å c: 184.524Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.273
Expression system: Escherichia coli