3f12

X-ray diffraction
2.95Å resolution

Germline V-genes sculpt the binding site of a family of antibodies neutralizing human cytomegalovirus

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
M2J1 Fab Chains: A, C
Molecule details ›
Chains: A, C
Length: 216 amino acids
Theoretical weight: 23.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
8f9 Fab Chains: B, D
Molecule details ›
Chains: B, D
Length: 242 amino acids
Theoretical weight: 25.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3221
Unit cell:
a: 87.86Å b: 87.86Å c: 241.85Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.3 0.3 0.32
Expression system: Escherichia coli