3f0p

X-ray diffraction
1.64Å resolution

Crystal structure of the mercury-bound form of MerB, the Organomercurial Lyase involved in a bacterial mercury resistance system

Released:

Function and Biology Details

Reaction catalysed:
An alkylmercury + H(+) = an alkane + Hg(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkylmercury lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77072 (Residues: 1-212; Coverage: 100%)
Gene name: merB
Sequence domains:
Structure domains: Beta-Lactamase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 38.372Å b: 89.203Å c: 51.585Å
α: 90° β: 100.48° γ: 90°
R-values:
R R work R free
0.186 0.184 0.213
Expression system: Escherichia coli