› 3eze

Solution NMR

COMPLEX OF THE AMINO TERMINAL DOMAIN OF ENZYME I AND THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE

Released:
DOI: 10.2210/pdb3eze/pdb
PDB 3eze coloured by chain and viewed from the front.
PDB 3eze coloured by chain and viewed from the side.
PDB 3eze coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Solution structure of the 40,000 Mr phosphoryl transfer complex between the N-terminal domain of enzyme I and HPr.
Garrett DS, Seok YJ, Peterkofsky A, Gronenborn AM, Clore GM
Nat. Struct. Biol. 6 166-73 (1999)
PMID: 10048929

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphoenolpyruvate-protein phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 28.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08839 (Residues: 1-258; Coverage: 45%)
Gene names: JW2409, b2416, ptsI
Sequence domains:
Structure domains:
Phosphocarrier protein HPr Chain: B
Molecule details ›
Chain: B
Length: 85 amino acids
Theoretical weight: 9.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA04 (Residues: 1-85; Coverage: 100%)
Gene names: JW2408, b2415, hpr, ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like

Ligands and Environments

1 bound ligand:
Ligand PO3 1 x PO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

21 review citations

Challenges in structural approaches to cell modeling.
Im et al. (2016)
20 more

2 mentions without citation

Structural alphabets derived from attractors in conformational space.
Pandini et al. (2010)
1 more