3exm

X-ray diffraction
1.65Å resolution

Crystal structure of the phosphatase SC4828 with the non-hydrolyzable nucleotide GPCP

Released:
Entry authors: Singer AU, Xu X, Zheng H, Joachimiak A, Edwards AM, Savchenko A, Yakunin AF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed:
A nucleoside diphosphate + H(2)O = a nucleoside phosphate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF402 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.51 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FBN7 (Residues: 1-236; Coverage: 100%)
Gene name: SCO5041
Sequence domains: Protein of unknown function (DUF402)
Structure domains: FomD-like

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 36.941Å b: 79.706Å c: 82.507Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.199
Expression system: Escherichia coli