› 3evp

X-ray diffraction
1.45Å resolution

crystal structure of circular-permutated EGFP

Released:
DOI: 10.2210/pdb3evp/pdb
PDB 3evp coloured by chain and viewed from the front.
PDB 3evp coloured by chain and viewed from the side.
PDB 3evp coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Structural basis for calcium sensing by GCaMP2.
Wang Q, Shui B, Kotlikoff MI, Sondermann H
Structure 16 1817-27 (2008)
PMID: 19081058

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.35 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-144; Coverage: 60%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 51.06Å b: 62.22Å c: 69.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.132 0.174
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Axon-Targeting Motifs: Mechanisms and Applications of Enhancing Axonal Localisation of Transmembrane Proteins.
Steele-Nicholson et al. (2022)
18 more

15 mentions without citation

Tracking RNA with light: selection, structure, and design of fluorescence turn-on RNA aptamers.
Trachman et al. (2019)
14 more