X-ray diffraction
1.45Å resolution

crystal structure of circular-permutated EGFP

Source organism: Aequorea victoria
Primary publication:
Structural basis for calcium sensing by GCaMP2.
Structure 16 1817-27 (2008)
PMID: 19081058

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.35 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
  • Canonical: P42212 (Residues: 2-144; Coverage: 60%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 51.06Å b: 62.22Å c: 69.45Å
α: 90° β: 90° γ: 90°
R R work R free
0.136 0.132 0.174
Expression system: Escherichia coli