3eve

X-ray diffraction
1.7Å resolution

Crystal structure of GpppA complex of yellow fever virus methyltransferase and S-adenosyl-L-homocysteine

Released:
Source organism: Yellow fever virus 17D
Primary publication:
Analysis of flavivirus NS5 methyltransferase cap binding.
J. Mol. Biol. 385 1643-54 (2009)
PMID: 19101564

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase NS5 Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.29 KDa
Source organism: Yellow fever virus 17D
Expression system: Escherichia coli
UniProt:
  • Canonical: P03314 (Residues: 2507-2772; Coverage: 8%)
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P65
Unit cell:
a: 104.422Å b: 104.422Å c: 51.746Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.207 0.228
Expression system: Escherichia coli