› 3esl

X-ray diffraction
1.74Å resolution

Crystal structure of the conserved N-terminal domain of the mitotic checkpoint component BUB1

Released:
DOI: 10.2210/pdb3esl/pdb
PDB 3esl coloured by chain and viewed from the front.
PDB 3esl coloured by chain and viewed from the side.
PDB 3esl coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of the N-terminal region of BUB1 provides insight into the mechanism of BUB1 recruitment to kinetochores.
Bolanos-Garcia VM, Kiyomitsu T, D'Arcy S, Chirgadze DY, Grossmann JG, Matak-Vinkovic D, Venkitaraman AR, Yanagida M, Robinson CV, Blundell TL
Structure 17 105-16 (2009)
PMID: 19141287

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Budding uninhibited by benzimidazole-related protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 24.38 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A6ZUJ9 (Residues: 29-230; Coverage: 20%)
Gene names: BUB1, SCY_2082
Sequence domains: Mad3/BUB1 homology region 1
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NHE 2 x NHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 130.304Å b: 59.784Å c: 71.299Å
α: 90° β: 97.96° γ: 90°
R-values:
R R work R free
0.19 0.19 0.216
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Bub1 kinase in the regulation of mitosis.
Kim et al. (2021)
14 more

6 mentions without citation

BUB1 and BUBR1: multifaceted kinases of the cell cycle.
Bolanos-Garcia et al. (2011)
5 more