X-ray diffraction
2.2Å resolution

Crystal structure of divergent enolase from Oceanobacillus Iheyensis complexed with Mg and L-malate.


Function and Biology Details

Reaction catalysed:
Galactarate = (2S,3R)-2,3-dihydroxy-5-oxohexanedioate + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo octamer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Galactarate dehydratase (D-threo-forming) Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 391 amino acids
Theoretical weight: 44.4 KDa
Source organism: Oceanobacillus iheyensis
Expression system: Escherichia coli
  • Canonical: Q8EMJ9 (Residues: 1-391; Coverage: 100%)
Gene name: OB2843
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P1
Unit cell:
a: 105.584Å b: 105.695Å c: 105.718Å
α: 109.31° β: 109.47° γ: 109.66°
R R work R free
0.226 0.224 0.241
Expression system: Escherichia coli