3err

X-ray diffraction
2.27Å resolution

Microtubule binding domain from mouse cytoplasmic dynein as a fusion with seryl-tRNA synthetase

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytoplasmic dynein 1 heavy chain 1; Serine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 536 amino acids
Theoretical weight: 60.96 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SJX7 (Residues: 94-419; Coverage: 77%)
  • Canonical: Q9JHU4 (Residues: 3260-3427; Coverage: 4%)
Gene names: Dhc1, Dnch1, Dnchc1, Dyhc, Dync1h1, TTHA0875, serS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: R3
Unit cell:
a: 144.302Å b: 144.302Å c: 159.946Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.197 0.247
Expression system: Escherichia coli