› 3emi

X-ray diffraction
1.8Å resolution

Crystal structure of Hia 307-422 non-adhesive domain

Released:
DOI: 10.2210/pdb3emi/pdb
PDB entry 3emi coloured by chain, front view.
PDB entry 3emi coloured by chain, side view.
PDB entry 3emi coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Repetitive architecture of the Haemophilus influenzae Hia trimeric autotransporter.
Meng G, St Geme JW, Waksman G
J Mol Biol 384 824-36 (2008)
PMID: 18948113

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175334 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adhesin Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 12.05 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q48152 (Residues: 307-422; Coverage: 11%)
Gene name: hia
Sequence domains: Tryptophan-ring motif of head of Trimeric autotransporter adhesin
Structure domains: Trimeric adhesin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6322
Unit cell:
a: 53.864Å b: 53.864Å c: 151.688Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.162 0.198
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Human pathogens utilize host extracellular matrix proteins laminin and collagen for adhesion and invasion of the host.
Singh et al. (2012)
11 more

4 mentions without citation

Recent advances in the understanding of trimeric autotransporter adhesins.
Kiessling et al. (2020)
3 more