3em1

X-ray diffraction
1.5Å resolution

Crystal structure of the mimivirus NDK +Kpn-N62L double mutant complexed with dTDP

Released:

Function and Biology Details

Reaction catalysed:
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside diphosphate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 16.63 KDa
Source organism: Acanthamoeba polyphaga mimivirus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5UQL3 (Residues: 2-92, 93-137; Coverage: 99%)
Gene names: MIMI_R418, NDK
Sequence domains: Nucleoside diphosphate kinase
Structure domains: Nucleoside diphosphate kinase-like domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P63
Unit cell:
a: 70.343Å b: 70.343Å c: 104.137Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.183 0.196
Expression system: Escherichia coli