X-ray diffraction
1.85Å resolution

Function and Biology Details

Reaction catalysed:
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Calmodulin-1; Green fluorescent protein; Myosin light chain kinase, smooth muscle, deglutamylated form Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 50.7 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
  • Canonical: P11799 (Residues: 1730-1763; Coverage: 2%)
  • Canonical: P0DP29 (Residues: 23-42, 45-149; Coverage: 84%)
  • Canonical: P42212 (Residues: 2-144, 151-170, 191-192; Coverage: 69%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1, GFP, Mylk
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 126.131Å b: 47.31Å c: 68.94Å
α: 90° β: 100.48° γ: 90°
R R work R free
0.191 0.189 0.241
Expression system: Escherichia coli BL21(DE3)