› 3ej2

X-ray diffraction
2.12Å resolution

Crystal structure of inorganic pyrophosphatase from burkholderia pseudomallei with bound 5-amino-1-(4-chlorophenyl)-1h-pyrazole-4-carbonitrile, H32 crystal form

Released:
DOI: 10.2210/pdb3ej2/pdb
PDB entry 3ej2 coloured by chain, front view.
PDB entry 3ej2 coloured by chain, side view.
PDB entry 3ej2 coloured by chain, top view.
Source organism: Burkholderia pseudomallei 1710b
Primary publication:
Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh L, Gallagher LA, Patrapuvich R, Clifton MC, Gardberg AS, Edwards TE, Armour B, Begley DW, Dieterich SH, Dranow DM, Abendroth J, Fairman JW, Fox D, Staker BL, Phan I, Gillespie A, Choi R, Nakazawa-Hewitt S, Nguyen MT, Napuli A, Barrett L, Buchko GW, Stacy R, Myler PJ, Stewart LJ, Manoil C, Van Voorhis WC
PLoS One 8 e53851 (2013)
PMID: 23382856

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174679 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 21.47 KDa
Source organism: Burkholderia pseudomallei 1710b
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JUV5 (Residues: 1-175; Coverage: 100%)
Gene names: BURPS1710b_1237, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
Ligand 928 1 x 928
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: R32
Unit cell:
a: 68.496Å b: 68.496Å c: 193.565Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.203 0.249
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The design and analysis of transposon insertion sequencing experiments.
Chao et al. (2016)
10 more

2 mentions without citation

The Seattle Structural Genomics Center for Infectious Disease (SSGCID).
Myler et al. (2009)
1 more