3ei1

X-ray diffraction
2.8Å resolution

Structure of hsDDB1-drDDB2 bound to a 14 bp 6-4 photoproduct containing DNA-duplex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA damage-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 1158 amino acids
Theoretical weight: 129.25 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q16531 (Residues: 1-1140; Coverage: 100%)
Gene names: DDB1, XAP1
Sequence domains:
Structure domains:
DNA damage-binding protein 2 Chain: B
Molecule details ›
Chain: B
Length: 383 amino acids
Theoretical weight: 43.56 KDa
Source organism: Danio rerio
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q2YDS1 (Residues: 60-423; Coverage: 73%)
Gene names: ddb2, si:dkey-45f10.3
Sequence domains: WD domain, G-beta repeat
Structure domains:
5'-D(*DAP*DCP*DGP*DCP*DGP*DAP*(64T)P*(5PY)P*DGP*DCP*DGP*DCP*DCP*DC)-3' Chain: G
Molecule details ›
Chain: G
Length: 14 nucleotides
Theoretical weight: 4.24 KDa
5'-D(*DTP*DGP*DGP*DGP*DCP*DGP*DCP*DAP*DAP*DTP*DCP*DGP*DCP*DG)-3' Chain: H
Molecule details ›
Chain: H
Length: 14 nucleotides
Theoretical weight: 4.32 KDa

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 112.74Å b: 123.6Å c: 158.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.278
Expression system: Trichoplusia ni