3efr

X-ray diffraction
2.55Å resolution

Biotin protein ligase R40G mutant from Aquifex aeolicus in complex with biotin

Released:

Function and Biology Details

Reaction catalysed:
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biotin--[biotin carboxyl-carrier protein] ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.65 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66837 (Residues: 1-233; Coverage: 100%)
Gene names: aq_566, birA
Sequence domains: Biotin/lipoate A/B protein ligase family
Structure domains:

Ligands and Environments


Cofactor: Ligand BTN 2 x BTN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: P212121
Unit cell:
a: 41.251Å b: 79.928Å c: 140.173Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.289
Expression system: Escherichia coli BL21(DE3)