› 3ec1

X-ray diffraction
2.36Å resolution

Structure of YqeH GTPase from Geobacillus stearothermophilus (an AtNOS1 / AtNOA1 ortholog)

Released:
DOI: 10.2210/pdb3ec1/pdb
PDB entry 3ec1 coloured by chain, front view.
PDB entry 3ec1 coloured by chain, side view.
PDB entry 3ec1 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
The structure of YqeH. An AtNOS1/AtNOA1 ortholog that couples GTP hydrolysis to molecular recognition.
Sudhamsu J, Lee GI, Klessig DF, Crane BR
J Biol Chem 283 32968-76 (2008)
PMID: 18801747

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CP-type G domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 369 amino acids
Theoretical weight: 41.12 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0VWU7 (Residues: 1-369; Coverage: 100%)
Gene names: B4114_2212, D9548_03520, yqeH
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 47.615Å b: 81.097Å c: 108.173Å
α: 90° β: 91.5° γ: 90°
R-values:
R R work R free
0.254 0.254 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Role of GTPases in bacterial ribosome assembly.
Britton RA. (2009)
4 more

4 mentions without citation

Structural classification of proteins and structural genomics: new insights into protein folding and evolution.
Andreeva et al. (2010)
3 more