X-ray diffraction
1.85Å resolution

Crystal structure of purine nucleoside phosphorylase from Schistosoma mansoni in complex with adenine


Function and Biology Details

Reaction catalysed:
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Purine nucleoside phosphorylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 287 amino acids
Theoretical weight: 31.2 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli DH5[alpha]
  • Canonical: Q9BMI9 (Residues: 1-287; Coverage: 100%)
Sequence domains: Phosphorylase superfamily
Structure domains: Rossmann fold

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 48.989Å b: 119.995Å c: 130.982Å
α: 90° β: 90° γ: 90°
R R work R free
0.164 0.162 0.201
Expression system: Escherichia coli DH5[alpha]