3e9c

X-ray diffraction
2Å resolution

Structure of a tryptic core fragment of TIGAR from Danio rerio

Released:
Source organism: Danio rerio
Entry authors: Li H, Jogl G

Function and Biology Details

Reaction catalysed:
Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-2,6-bisphosphatase TIGAR B Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 29.61 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7ZVE3 (Residues: 1-257; Coverage: 100%)
Gene names: si:ch211-240j22.3, tigarb, zgc:56074
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P65
Unit cell:
a: 136.672Å b: 136.672Å c: 66.753Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.17 0.209
Expression system: Escherichia coli