X-ray diffraction
2.3Å resolution

H. influenzae beta-carbonic anhydrase, variant Y181F with 1M bicarbonate


Function and Biology Details

Reaction catalysed:
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Carbonic anhydrase 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 229 amino acids
Theoretical weight: 26.27 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
  • Canonical: P45148 (Residues: 1-229; Coverage: 100%)
Gene names: HI_1301, can
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 250.141Å b: 145.225Å c: 53.499Å
α: 90° β: 93.78° γ: 90°
R R work R free
0.207 0.205 0.242
Expression system: Escherichia coli