3e2u

X-ray diffraction
2.6Å resolution

Crystal structure of the zink-knuckle 2 domain of human CLIP-170 in complex with CAP-Gly domain of human dynactin-1 (p150-GLUED)

Released:
Source organism: Homo sapiens
Primary publication:
Structure-function relationship of CAP-Gly domains.
Nat. Struct. Mol. Biol. 14 959-67 (2007)
PMID: 17828277

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dynactin subunit 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 97 amino acids
Theoretical weight: 10.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14203 (Residues: 18-111; Coverage: 7%)
Gene name: DCTN1
Sequence domains: CAP-Gly domain
Structure domains: CAP Gly-rich-like domain
CAP-Gly domain-containing linker protein 1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 42 amino acids
Theoretical weight: 4.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30622 (Residues: 1399-1438; Coverage: 3%)
Gene names: CLIP1, CYLN1, RSN
Sequence domains: CLIP1 zinc knuckle

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 116.22Å b: 116.31Å c: 79.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.238
Expression system: Escherichia coli